Abstract: Objective　To investigate outbreak of Achromobacter xylosoxidans in neonatesand present clinical and laboratory data of the patients. Methods　All the neonates with bacteremia due to Achromobacter xylosoxidans from Mar 2012 to Dec 2013 were retrospectively analyzed. Results　Eighteen episodes of bacteremia occurred in 13 neonates. All neonates were preterm newborns and 61.5% (8/13) had birth weight<1000 g. Prematurity, total parenteral nutrition, previous broad spectrum antibiotic therapy and umbilical venous catheter use were predisposing factors. Prematurity and respiratory distress syndrome were the most common diagnosis at admission. The frequent features were thrombocytopenia, abdominal distention, neutropenia and apnea. Three (23.1%) episodes were breakthrough infections. All isolates were susceptible to Trimethoprim-sulfametoxazole and Meropenem, but showed varied susceptibility to other antibiotics. Conclusions　Achromobacter xylosoxidans has potential risk of serious infections in neonate, inviting close attention to early diagnosis. Timely and accurate susceptibility testing and consideration for predisposing factors could help to control neonatal bacteremia.